Tannic acid (Gallotannic acid) is a novel hERG channel blocker.

CXCL12 cells:7.5 μg/ml, A549 cells (48 h):20-40 µM, A549 cells (24 h):40-60 µM, α-amylase:3.46 μg/mL, tyrosine kinase activity of epidermal growth factor receptor (EGFr):323 nM, ERG channel (human):3.4 μM

METHODS: Rat cardiomyocytes H9C2 were treated with Tannic acid (5-50 µM) for 24 h. Cell viability was determined by CCK-8 Assay.
RESULTS: Treatment of H9C2 cells with Tannic acid concentration ≥ 30 µM resulted in a significant decrease in cell viability. [1]
METHODS: Human lung cancer cells A549 were treated with Tannic acid (20-60 µM) for 24-48 h. Cell cycle was measured by Flow cytometry.
RESULTS: Tannic acid inhibited cell viability in a dose-dependent manner at 24 h, with higher inhibition after 48 h. The IC50 values for Tannic acid were estimated to be in the range of 40-60 µM at 24 h and 20-40 µM at 48 h. [1] METHODS: Human lung cancer cells A549 were treated with Tannic acid (20-60 µM) for 24-48 h. Cell cycle was measured by Flow cytometry. [2]

METHODS: To investigate the effects on oxidative stress mice, Tannic acid (2.5-10 mg/kg) was administered orally to diquat-induced oxidative stress C57BL/6J mouse model once daily for ten days.
RESULTS: Tannic acid pretreatment in the oxidative stress mouse model failed to alter antioxidant enzymes but modulated jejunal morphology, colon length, antioxidant pathways, and intestinal barrier in the diquat oxidative model. [3]